HPLC Column, Axis C18, 5um, 4.6mm ID x 150mm Length, 100A. Cogent Brand. 1 EA.

Additional Info:

The 59021‑15P Cogent Axis C18™ HPLC Column is a proven, legacy reversed‑phase C18 column, trusted by laboratories for consistent performance and method reliability across a wide range of analytical applications.

Built with a fully double end‑capped C18 bonded phase on high‑purity silica, this column minimizes secondary interactions and ensures reproducible retention and peak shape, making it ideal as a direct replacement or alternate in USP L1 methods.

✅ Widely used as a standard reference column for USP L1 applications

150 mm Length – High Resolution for Analytical Methods

  • Provides strong resolving power for complex mixtures

  • Improves separation of closely eluting analytes

  • Increases peak capacity for method development and validation

  • Ideal for routine QC and regulated workflows

✅ Selected when reliable resolution and reproducibility are required

4.6 mm ID – Standard Analytical Platform

  • Compatible with standard HPLC systems

  • Ideal for:

    • routine analytical testing

    • method validation and transfer

Typical Flow Rate Range

  • ~0.8–1.5 mL/min

✅ Provides robust and consistent analytical performance

5 µm Particle – Balanced Performance & Low Backpressure

  • Provides:

    • Reliable efficiency for routine HPLC methods

    • Lower backpressure compared to smaller particle sizes

    • Long column lifetime

✅ Ideal for:

  • Routine QC and validated methods

  • Method transfer between laboratories

100 Å Pore Size – Standard Small Molecule Separations

  • Optimized for:

    • small molecule analysis

    • traditional reversed‑phase methods

  • Provides stable performance for:

    • hydrophobic compounds

    • neutral analytes

✅ Ensures consistent chromatography across standard applications

Why Choose Axis C18™ Columns

Proven Legacy Performance

  • Widely used in analytical laboratories for many years

  • Trusted for repeatability and consistency

USP L1 Compatibility

  • Fully suitable for USP L1 methods

  • Ideal for method validation and transfer

Reliable Selectivity

  • Traditional C18 retention characteristics

  • Predictable behavior across common reversed‑phase methods

✅ Preferred for routine methods requiring dependable performance

Application Performance Advantages

  • Suitable for:

    • Hydrophobic compounds

    • Neutral analytes

    • Long‑chain fatty acids

  • Provides:

    • Longer retention than C8 phases

    • Stable and reproducible separations

✅ Ideal for general‑purpose HPLC analysis

System & Sample Considerations

  • Compatible with standard HPLC systems

  • Suitable for routine and high‑throughput workflows

Recommended sample preparation:

  • 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

  • Prevent column fouling

  • Maintain long‑term performance and reproducibility

Typical Applications

  • USP L1 analytical methods

  • Pharmaceutical QC and validation

  • Routine HPLC analysis

  • Method development and transfer

  • Hydrophobic compound separations

  • Long‑chain fatty acid analysis

Important Usage Notes

  • Designed for standard analytical HPLC systems (4.6 mm ID)

  • Suitable for general reversed‑phase chromatography

  • Ideal for USP L1 method compatibility and reproducibility

  • Monitor system pressure according to instrument capability

 

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